FastQCFastQC Report
Thu 2 Jun 2016
DRR015266_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015266_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10444762
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT513040.49119357626339405No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430780.41243639634871526No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT366420.3508169932450351No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT270230.25872298478414346RNA PCR Primer, Index 39 (95% over 23bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG244910.23448116864702137RNA PCR Primer, Index 39 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216090.20688839056361455No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT146820.14056806655814658RNA PCR Primer, Index 39 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA263600.056.761421
GTATCAA491150.042.0664521
ATCAACG636800.032.2702033
TCAACGC643150.031.9303684
TATCAAC648350.031.750832
CAACGCA669050.030.7714465
AACGCAG693750.029.7086646
GTGGTAT103600.028.6031381
TGGTATC106700.027.4213332
TATAACG7450.026.8743762
ACGCAGA778450.026.2190197
CGCAGAG783950.025.8365148
GCAGAGT815350.024.3337559
GTACATG541200.023.0050931
TACATGG524450.022.714732
GAGTACT471950.021.46362912-13
ACATGGG558100.020.786573
CAGAGTA796600.020.72621710-11
AGTACTT473950.020.66270312-13
GTACTTT487000.020.50772314-15
Produced by FastQC (version 0.11.5)