FastQCFastQC Report
Thu 2 Jun 2016
DRR015265_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015265_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7879041
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357310.4534942767781003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303830.3856179958956934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181060.22979954032476793No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA210400.055.3885081
GTATCAA386550.040.7052271
ATCAACG499800.031.3814833
TCAACGC504300.031.1240564
TATCAAC511950.030.6096882
CAACGCA534500.029.2547865
AACGCAG549850.028.5124176
GTGGTAT83800.027.8894881
ACGCAGA604900.025.655077
TGGTATC89300.025.4634382
CGCAGAG612500.025.2041538
GCAGAGT634600.023.9683979
TACCTGG52050.022.9836882
GTACATG443200.022.906021
TACATGG428000.022.3607642
GAGTACT359600.020.92755512-13
CAGAGTA614550.020.7709210-11
ACATGGG452950.020.4387863
AGTACTT359250.020.39068212-13
GCCGTAT22550.020.37823190-91
Produced by FastQC (version 0.11.5)