FastQCFastQC Report
Thu 2 Jun 2016
DRR015265_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015265_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7879041
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421290.5346970525981525No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT374110.4748166686783328No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367050.46585618732025885No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216550.2748430932140092No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT201360.2555640972042156TruSeq Adapter, Index 1 (95% over 21bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG133050.16886572870987726TruSeq Adapter, Index 1 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT110590.14035972144325687RNA PCR Primer, Index 1 (95% over 23bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92520.11742545825056629No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA239050.055.060081
GTATCAA434650.040.222341
ATCAACG556400.031.3472083
TCAACGC560000.031.1700614
TATCAAC568000.030.8402542
GTGGTAT94350.030.769891
CAACGCA575300.030.3520895
AACGCAG592050.029.524126
TGGTATC100600.028.357972
ACGCAGA659500.026.2424097
CGCAGAG668250.025.7822348
TATAACG8700.025.103112
GCAGAGT692550.024.4243169
TAACGCA8700.024.0531794
TACCTGG56700.023.0305542
GTACATG459850.022.7256491
TACATGG450150.022.2973792
GAGTACT402100.022.12056412-13
CAGAGTA675000.021.265410-11
GTACTTT414300.021.15706814-15
Produced by FastQC (version 0.11.5)