FastQCFastQC Report
Thu 2 Jun 2016
DRR015264_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015264_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8526644
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488070.5724057436900145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT417890.49009903544700584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239790.28122435978328636No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA111900.13123568897681198No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA111320.13055546824753092No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100600.11798311269943954No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA87890.10307689637329763No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA306950.054.7473221
GTATCAA580500.042.2515031
ATCAACG742700.033.0124363
TATCAAC741550.032.990372
TCAACGC759200.032.2997064
CAACGCA826850.029.6240255
AACGCAG850150.028.9192076
CGCAGAG892350.027.0678188
ACGCAGA900450.027.031177
GTGGTAT123700.026.749461
TGGTATC125600.026.059942
GCAGAGT921600.025.6676589
GTACATG659850.023.7382551
CATGGGT113050.023.555544
TACATGG641600.023.4370612
GAGTACT495350.023.0323212-13
GCCGTAT30700.022.8231790-91
ACATGGG664250.022.0563413
GTACTTT513100.022.02702914-15
CGTATCA15950.021.67936390-91
Produced by FastQC (version 0.11.5)