FastQCFastQC Report
Thu 2 Jun 2016
DRR015264_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015264_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8526644
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT718010.8420780790191311No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT551030.6462448766478347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT476250.5585433143450108No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT383310.4495438064495246RNA PCR Primer, Index 27 (95% over 23bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG317370.37220974629643266RNA PCR Primer, Index 27 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274720.322190066807058No Hit
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT202540.23753776984239053RNA PCR Primer, Index 27 (96% over 25bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117040.1372638519914752No Hit
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCT97970.11489866353045818RNA PCR Primer, Index 27 (96% over 28bp)
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA88610.1039213083130948No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA328200.054.560281
GTATCAA600650.040.4149251
TATCAAC776050.031.2444952
ATCAACG778700.031.0684243
TCAACGC792050.030.5332784
CAACGCA833050.029.0928155
GTGGTAT132050.028.6538731
AACGCAG859300.028.2464436
TGGTATC134450.027.6151142
ACGCAGA942250.025.532867
CGCAGAG937350.025.4665858
GCAGAGT971850.024.1505899
GTACATG685000.023.6920761
TACATGG665400.023.461482
TACCTGG58450.023.4314652
CATGGGT111350.022.6339654
GAGTACT530850.022.55702612-13
ACATGGG686800.022.24293
GTACTTT552250.021.51156814-15
AGTACTT529850.021.50911712-13
Produced by FastQC (version 0.11.5)