FastQCFastQC Report
Thu 2 Jun 2016
DRR015263_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015263_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7556677
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341310.45166678422274764No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291030.38512960127844553No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173720.22988940773834848No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA212700.053.498061
GTATCAA390600.041.4020771
ATCAACG505200.031.8827463
TCAACGC507750.031.7197534
TATCAAC509950.031.665892
CAACGCA536350.030.0368355
AACGCAG553450.029.2732896
GTGGTAT88100.027.8215681
ACGCAGA609300.026.2536267
CGCAGAG614400.025.9330628
TGGTATC94300.025.4658552
GCAGAGT634150.024.6171469
TACCTGG55300.024.189492
GTACATG436750.023.639751
TACATGG424200.023.1716822
GAGTACT355350.021.5814912-13
ACATGGG449150.021.1784083
TATAACG7200.020.9250092
CAGAGTA610000.020.86648210-11
GTACTTT363300.020.79564714-15
Produced by FastQC (version 0.11.5)