FastQCFastQC Report
Thu 2 Jun 2016
DRR015263_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015263_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7556677
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400640.5301801307638265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT347450.45979204880663815No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT340850.45105805104545293No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206500.2732682632855685No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT186200.24640460350495327RNA PCR Primer, Index 26 (100% over 22bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG145820.19296841720242905RNA PCR Primer, Index 26 (100% over 29bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT100680.1332331658478985RNA PCR Primer, Index 26 (100% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88000.11645330348246988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA238100.054.6148571
GTATCAA418900.039.63851
ATCAACG544050.030.3183773
TCAACGC547550.030.0581134
TATCAAC550550.030.0496142
CAACGCA568400.028.9747895
AACGCAG583650.028.2800776
GTGGTAT92850.028.2263261
TGGTATC97600.026.4329132
ACGCAGA650200.025.2245887
CGCAGAG660150.024.6648868
GCAGAGT685700.023.3610279
TACCTGG52500.023.2266882
GTACATG446900.022.8763831
TACATGG437550.022.2534772
GAGTACT395700.021.76451112-13
GTACTTT405300.021.03613714-15
CAGAGTA662650.020.8614110-11
ACATGGG456250.020.5889453
AGTACTT394450.020.31678612-13
Produced by FastQC (version 0.11.5)