FastQCFastQC Report
Thu 2 Jun 2016
DRR015262_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015262_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10058910
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTCGCCGT466900.4641656004477622Illumina Single End PCR Primer 1 (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421830.41935955287401916No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC398480.3961463021341279Illumina Single End PCR Primer 1 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359250.3571460526041092No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG339500.3375117184665138No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198150.19698953465136876No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA133370.1325889186800558No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG128140.1273895481717204No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA125890.12515272529528548No Hit
CATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTCGCCGG122610.12189193461319368Illumina Single End PCR Primer 1 (96% over 25bp)
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT120410.11970481891179065No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA115290.1146148041885254No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA114130.1134615977277856No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA104940.10432541895692476No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA103140.10253596065577682No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA102890.10228742478061738No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT101630.10103480396981383No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA280000.053.7071
GTATCAA538750.041.949511
ATCAACG690350.032.7538073
TCAACGC707450.031.9866564
TATCAAC728900.030.9132562
GCTCTAG12500.029.9390031
CAACGCA788300.028.7175755
AACGCAG805450.028.1566946
GTGGTAT111200.027.6623211
ACGCAGA830950.026.9056427
CGCAGAG829700.026.8151048
TGGTATC114900.026.4601172
GCAGAGT864900.025.293179
CATGGGT99250.025.088674
GTACATG656150.024.8312511
TACATGG605200.024.7859862
TACCTGG39000.023.9953352
ACATGGG625650.023.3373973
CCGGATC49050.023.32824546-47
TATAACG9200.022.3286382
Produced by FastQC (version 0.11.5)