FastQCFastQC Report
Thu 2 Jun 2016
DRR015262_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015262_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10058910
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT859940.8549037619384208No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT491520.4886414134334635No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT486550.4837005202352939RNA PCR Primer, Index 17 (95% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421180.4187133595986046No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG345010.3429894491550277RNA PCR Primer, Index 17 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT264460.26291119017865755RNA PCR Primer, Index 17 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT234480.233106768029538No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA188160.18705804107999774TruSeq Adapter, Index 25 (96% over 27bp)
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG150920.1500361371162482No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT125210.12447670771485182No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT122380.121663281608047RNA PCR Primer, Index 17 (96% over 26bp)
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA120950.12024165640213502No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA116280.11559900625415676No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA107260.10663183187840432No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105320.10470319348716708No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA105300.10468331061715434No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGA104020.10341080693633803No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT27350.063.373559
AATCGTA21450.060.4446648
AACTCGT36250.056.725937
GGTATCA306800.055.6754261
AAACTCG39700.055.2341586
AAATCGT27000.049.537587
GTATCAA586700.041.3517571
ACTCGTA52500.038.473718
ATATCGT11500.037.9773457
GAAAACT70950.033.5979544
AAAATCG45700.033.0501256
ATCAACG751350.032.2654233
TCAACGC768700.031.4898284
CTCGGAT9150.031.3227449
AATATCG15750.030.9068266
TATCAAC794650.030.4960372
CAGAAAA154350.029.7737052
CAACGCA818800.029.5753465
AACGTAT6850.029.22159
AACGCAG839100.028.9194816
Produced by FastQC (version 0.11.5)