FastQCFastQC Report
Thu 2 Jun 2016
DRR015261_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015261_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9127505
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295860.3241411535792092No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248930.27272513134750404No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140660.15410564004073402No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA208200.054.2005771
GTATCAA387500.042.197821
ATCAACG496100.032.6577223
TCAACGC499850.032.272354
TATCAAC509950.031.8668612
CAACGCA538350.030.0404935
AACGCAG555900.029.149426
GTGGTAT84500.027.8759161
ACGCAGA606900.026.372167
TGGTATC88500.026.1063482
CGCAGAG617350.025.6984278
GCAGAGT639200.024.138119
GTACATG444200.022.9069711
TACATGG434950.022.2763962
GAGTACT351950.020.58783512-13
CAGAGTA618900.020.37542310-11
ACATGGG462000.020.2824063
AGAGTAC599450.020.09533510-11
AGTACTT360300.020.0349412-13
GTACTTT367450.019.4464914-15
Produced by FastQC (version 0.11.5)