FastQCFastQC Report
Thu 2 Jun 2016
DRR015261_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015261_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9127505
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT505330.5536343173736963No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT348530.3818458603966801No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300010.3286878506229249No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT265680.29107625796972997TruSeq Adapter, Index 11 (95% over 21bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG231510.2536399596603891TruSeq Adapter, Index 11 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172200.1886605375729731No Hit
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT145570.15948498521775664RNA PCR Primer, Index 11 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA226650.054.5789951
GTATCAA420050.041.7872621
ATCAACG538450.032.3412863
TCAACGC542550.032.0717284
TATCAAC549750.031.7237992
CAACGCA566250.030.7632085
AACGCAG582100.029.9411896
GTGGTAT89150.028.2732141
ACGCAGA646600.026.7363667
TGGTATC93950.026.152222
CGCAGAG656300.026.0978788
GCAGAGT681200.024.6497219
GTACATG446350.023.4561561
TACATGG434400.022.990922
GAGTACT397800.021.68408612-13
ACATGGG457850.021.262843
CAGAGTA663850.020.78201910-11
GTACTTT411000.020.7177714-15
AGAGTAC639650.020.61876710-11
AGTACTT405150.020.56080212-13
Produced by FastQC (version 0.11.5)