FastQCFastQC Report
Thu 2 Jun 2016
DRR015260_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015260_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8974386
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291050.3243118805007941No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245590.2736566044741111No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133170.14838898170860937No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA207050.054.7726971
GTATCAA380700.041.289721
ATCAACG479350.032.642223
TCAACGC485800.032.2223324
TATCAAC495700.031.4610942
CAACGCA521300.030.0804125
AACGCAG536300.029.2560566
GTGGTAT81450.029.0353091
TGGTATC85300.026.661362
ACGCAGA587650.026.3999027
CGCAGAG590600.026.021978
GCAGAGT615650.024.4098789
GTACATG418350.022.7756331
TACATGG402250.022.297052
GAGTACT347950.021.61458612-13
GCCGTAT22450.021.1787690-91
CAGAGTA600650.020.81546810-11
ACATGGG421950.020.6554363
GTACTTT361800.020.53553814-15
AGAGTAC577900.019.77688210-11
Produced by FastQC (version 0.11.5)