FastQCFastQC Report
Thu 2 Jun 2016
DRR015260_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015260_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8974386
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT478840.5335629646418151No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT337080.37560229747193846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292360.32577159039069636No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT277990.3097593528961201TruSeq Adapter, Index 3 (95% over 21bp)
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG246020.2741357458883538TruSeq Adapter, Index 3 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158080.17614575526392556No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT152840.17030691570431672RNA PCR Primer, Index 3 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA221750.057.3347471
GTATCAA405100.040.97241
ATCAACG509700.032.3461343
TCAACGC514350.032.062554
TATCAAC529600.031.1878132
CAACGCA537600.030.8473995
GTGGTAT81250.030.185051
AACGCAG554600.029.9346566
TGGTATC86850.027.4528682
ACGCAGA623850.026.2836047
CGCAGAG625600.025.8886458
GCAGAGT650200.024.3845279
GTACATG418000.022.8803271
TACATGG394200.022.5660952
GAGTACT383550.022.10405512-13
CAGAGTA633700.021.47693410-11
GTACTTT390950.021.4367814-15
ACATGGG415950.020.6604423
AGAGTAC610950.019.75225410-11
AGTACTT384350.019.73209812-13
Produced by FastQC (version 0.11.5)