FastQCFastQC Report
Thu 2 Jun 2016
DRR015259_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015259_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7074847
Sequences flagged as poor quality0
Sequence length97
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT393580.5563088502125912No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320020.452334870280587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176880.25001247376798397No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA170590.2411218221397579No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA155470.21975033523693163No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA132340.18705704872486995No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT108370.15317645738487348No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA104750.1480597389597259No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC104060.1470844528510652No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC96470.13635630565579723No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG95570.1350841933401528No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA89470.1264620987563406No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA84320.1191827893945975No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA77470.10950060121441496No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG73990.10458176692725651No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73800.1043132098828427No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC73330.10364888456245061No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA261750.055.5714841
GTATCAA502750.042.8768161
TATCAAC648250.033.1547782
ATCAACG650200.033.0431863
TCAACGC663450.032.3004954
CAACGCA756700.028.3621335
AACGCAG772950.027.7776476
CGCAGAG762650.027.746718
TCTGTCG19500.027.5320058
ACGCAGA777950.027.3524937
GCAGAGT790000.026.389669
GTGGTAT99250.025.6142311
TGGTATC102100.024.8600082
GTACATG573650.024.2745881
TACATGG561050.024.1335832
AATGCGG29850.023.9296597
GCGTAAT2703.6379788E-1223.6657851
GAGTACT434950.023.13414212-13
ACATGGG588850.022.5824183
CGTATCA16500.021.92182490-91
Produced by FastQC (version 0.11.5)