FastQCFastQC Report
Thu 2 Jun 2016
DRR015259_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015259_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7074847
Sequences flagged as poor quality0
Sequence length97
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT1014451.433882598450539No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT537910.7603132618981019RNA PCR Primer, Index 31 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT403610.5704858352413841No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338420.478342499844873No Hit
ACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCT291860.4125318893822015RNA PCR Primer, Index 31 (95% over 24bp)
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG274650.38820627499082316RNA PCR Primer, Index 31 (96% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188200.2660128197825338No Hit
CATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCT133690.1889652171983366RNA PCR Primer, Index 31 (96% over 27bp)
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA123080.1739684264550173No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT111250.15724721679493564No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG110270.1558620278290117No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG106920.15112694309855748No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG102060.14425753659407758No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA99450.14056841087870875No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG95740.13532448122199675No Hit
TTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTC95690.13525380831557204No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT95450.13491457836473353No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT94060.13294987156612714No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT90280.12760699984042057No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA87300.12339489461750905No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG84100.11887182860632887No Hit
CACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTG82990.11730289008370075RNA PCR Primer, Index 38 (95% over 24bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78160.11047588732307567No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG77270.10921790958871619No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC76570.10822848889877053No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC76480.10810127766720609No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG73720.10420013323256319No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT71350.10085023746803287No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA270650.055.9607281
GTATCAA512750.042.125211
TATCAAC657300.032.788872
ATCAACG662150.032.5038033
TCAACGC676050.031.8153174
CAACGCA730250.029.4929945
AACGCAG748050.028.8216116
TCTGTCG20000.028.2060058
CGCAGAG778950.027.2336438
ACGCAGA792350.026.9805367
GTGGTAT100250.026.8697811
GCAGAGT809350.025.8905059
TGGTATC105150.025.398942
GTCTCGC19600.025.3472881
GTACATG591750.024.3779131
TACATGG580800.024.3154242
TCGATTA19900.023.1328621
GAGTACT445650.022.97002812-13
ACATGGG612550.022.6440183
TACCTGG36450.022.467862
Produced by FastQC (version 0.11.5)