FastQCFastQC Report
Thu 2 Jun 2016
DRR015258_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015258_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8630504
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321890.37296778959838267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265940.3081395941650685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156130.18090484634501067No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA217000.054.690421
GTATCAA401200.043.3480531
ATCAACG524500.032.890633
TCAACGC527000.032.7288134
TATCAAC531500.032.564442
CAACGCA558550.030.8719585
AACGCAG570750.030.2606146
ACGCAGA626750.027.2784217
CGCAGAG636850.026.632428
GTGGTAT83900.025.7136021
GCAGAGT662000.025.0646889
GTACATG453650.023.787981
TACATGG440400.023.5555212
TGGTATC87300.023.46292
ACATGGG463700.021.723153
GAGTACT364750.021.59179712-13
CAGAGTA641500.020.9398710-11
AGTACTT365450.020.89060412-13
AGAGTAC615900.020.8166810-11
TACCTGG68300.020.3049742
Produced by FastQC (version 0.11.5)