FastQCFastQC Report
Thu 2 Jun 2016
DRR015258_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015258_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8630504
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369250.4278429162422033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314510.3644167246779562No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT296140.3431317568475723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183140.2122008170090646No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT155830.1805572420799527RNA PCR Primer, Index 23 (95% over 23bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG133140.15426677283273377RNA PCR Primer, Index 23 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCT90870.10528933188606368RNA PCR Primer, Index 23 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA223700.056.2536931
GTATCAA424150.043.035111
ATCAACG549550.032.980983
TATCAAC553950.032.8751682
TCAACGC551700.032.8396264
CAACGCA574600.031.563085
AACGCAG592350.030.7018156
ACGCAGA664300.027.1843247
CGCAGAG676150.026.478648
GTGGTAT87650.025.6543061
GCAGAGT698100.025.0711429
TGGTATC90350.024.1875382
GTACATG440750.022.497411
TACATGG432400.022.2059562
GAGTACT407400.021.93569412-13
CAGAGTA675500.021.09306110-11
AGTACTT409650.020.68722312-13
GTACTTT429200.020.58418714-15
AGAGTAC651000.020.57577110-11
ACATGGG458550.020.2743823
Produced by FastQC (version 0.11.5)