FastQCFastQC Report
Thu 2 Jun 2016
DRR015257_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015257_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11055470
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT385120.3483524445365055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT317910.28755900925062433No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179020.16192889130900812No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA382500.037.9299471
GTATCAA609500.034.022031
ATCAACG746350.027.532983
TCAACGC748800.027.402334
TATCAAC766350.026.8685722
CAACGCA783850.026.1247715
AACGCAG802050.025.5887016
GTACATG572800.023.983341
TACATGG548250.023.599062
ACGCAGA873500.023.2763217
CGCAGAG885750.022.8367888
GCAGAGT912850.021.8003759
ACATGGG580000.021.6580353
GAGTACT452250.021.28769112-13
GTACTTT450500.021.11794914-15
TACCTGG76150.020.6016752
CATGGGG337000.020.1785584
AGTACTT443000.020.0786512-13
AGAGTAC754750.019.83245310-11
CAGAGTA896900.018.37587410-11
Produced by FastQC (version 0.11.5)