FastQCFastQC Report
Thu 2 Jun 2016
DRR015257_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015257_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11055470
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421130.38092455589857327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363910.32916737144599006No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT245950.22246905830326527No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202230.18292302362540896No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT124790.11287625039912369RNA PCR Primer, Index 27 (95% over 23bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT114810.1038490448619552No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG113550.10270933754964737RNA PCR Primer, Index 27 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA402450.037.0121081
GTATCAA644600.033.9193341
ATCAACG791200.027.4778793
TCAACGC794050.027.371544
TATCAAC812200.026.952372
CAACGCA818700.026.486745
AACGCAG838100.025.873876
GTACATG565600.023.3683781
ACGCAGA923050.023.3441837
TACATGG544550.023.013022
CGCAGAG932600.023.0032398
TACCTGG77350.021.8332672
GCAGAGT964750.021.8286539
GAGTACT491150.021.35738412-13
ACATGGG577150.020.987533
GTACTTT496600.020.87688314-15
AGTACTT482850.020.6498312-13
AGAGTAC793400.020.33704810-11
TATAACG8300.019.7437462
CATGGGG336750.018.6258164
Produced by FastQC (version 0.11.5)