FastQCFastQC Report
Thu 2 Jun 2016
DRR015256_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015256_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9827704
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357330.36359458933643096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283290.2882565449671663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166440.16935797008131298No Hit
CATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTCGCCGT157350.1601086072596407Illumina RNA PCR Primer (96% over 25bp)
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC106020.10787870696960349No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA234100.053.2260861
GTATCAA439200.042.2231141
ATCAACG563450.032.5947343
TCAACGC568200.032.3324054
TATCAAC574100.032.0784952
CAACGCA613100.030.0165315
AACGCAG630350.029.2674666
ACGCAGA676000.026.9200387
CGCAGAG680800.026.5645288
GTACATG474350.025.2488171
GTGGTAT102750.024.9015431
GCAGAGT717450.024.8591129
TACATGG461650.024.447232
TGGTATC105700.023.0035572
ACATGGG489100.022.5762833
GAGTACT412000.021.69983912-13
CAGAGTA699550.020.69523610-11
AGAGTAC658950.020.58251810-11
GTACTTT433400.020.41851214-15
AGTACTT417550.020.41438912-13
Produced by FastQC (version 0.11.5)