FastQCFastQC Report
Thu 2 Jun 2016
DRR015256_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015256_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9827704
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT403540.41061472750909056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333780.3396317186598212No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT251960.2563772779481352No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197140.20059619215230742No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT139560.14200671896508074RNA PCR Primer, Index 17 (95% over 21bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG104620.10645416264063305RNA PCR Primer, Index 17 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA247250.054.5044821
GTATCAA465700.042.2165571
AATCGTA10350.038.686118
ACTCGTA20750.033.7687848
ATCAACG597100.032.6983033
TCAACGC604050.032.2662124
TATCAAC614200.031.9139422
ATCGTAT16000.031.0066019
CAACGCA631600.030.9537265
AACGCAG649750.030.1454226
AAACTCG23500.029.636026
ACGCAGA719500.027.0581517
CGCAGAG730100.026.5173428
GTGGTAT103100.025.3016241
GCAGAGT769700.024.7292089
AAATCGT16550.024.4783257
GTACATG478900.023.654721
TGGTATC107600.023.4799392
TACATGG466300.022.9315722
AACTCGT30350.022.7968377
Produced by FastQC (version 0.11.5)