FastQCFastQC Report
Thu 2 Jun 2016
DRR015255_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015255_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9810758
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT357090.3639779923223058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292180.2978159281882195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172590.17591912877679788No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA227500.055.35551
GTATCAA425800.042.1516721
ATCAACG547550.032.502673
TCAACGC554650.032.1453674
TATCAAC571050.031.1580732
CAACGCA599050.029.8084265
AACGCAG615250.029.0680856
ACGCAGA659850.026.7231397
CGCAGAG667200.026.3755478
GTGGTAT90800.026.1212081
TGGTATC91400.025.1573282
GTACATG465700.024.9360661
GCAGAGT693750.024.901179
TACATGG443900.024.2758752
ACATGGG464850.022.5500323
GAGTACT420200.021.28151112-13
CAGAGTA685600.020.88694210-11
AGAGTAC656600.020.70079210-11
AGTACTT421450.020.50049612-13
GTACTTT430400.020.48138814-15
Produced by FastQC (version 0.11.5)