FastQCFastQC Report
Thu 2 Jun 2016
DRR015255_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015255_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9810758
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT404310.41210882991915604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335660.3421346240524942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199780.2036336030304692No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT156980.1600080238448446No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA241300.055.343381
GTATCAA456250.041.975411
ATCAACG582150.032.5705223
TCAACGC589300.032.156454
TATCAAC607950.031.3904882
CAACGCA620000.030.5794935
AACGCAG641600.029.642246
GTGGTAT98800.027.9286791
TGGTATC98500.027.3146632
ACGCAGA708250.026.640527
CGCAGAG712450.026.324438
GCAGAGT742350.024.8692659
GTACATG474600.024.3613971
TACATGG455500.023.796642
ACATGGG478500.021.8536363
GAGTACT452500.021.68948712-13
GTACTTT459250.021.0112114-15
CAGAGTA732900.020.88056210-11
AGAGTAC701050.020.67346810-11
AGTACTT449750.020.5366712-13
Produced by FastQC (version 0.11.5)