FastQCFastQC Report
Thu 2 Jun 2016
DRR015254_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015254_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8684710
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT377100.4342113899024838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314030.36158950615507024No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186090.2142731305938828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA225650.057.8480031
GTATCAA442850.043.5753021
ATCAACG568600.033.7032743
TCAACGC574950.033.3406874
TATCAAC588850.032.594732
CAACGCA620550.030.8466915
AACGCAG634850.030.2094086
GTGGTAT87450.029.5196131
TGGTATC87200.029.0896072
ACGCAGA687750.027.6767487
CGCAGAG690750.027.3931568
GCAGAGT722950.025.6822099
GTACATG476750.024.4142861
TACATGG460000.023.65432
GAGTACT430900.021.91399412-13
ACATGGG485100.021.8823573
CAGAGTA709200.021.30130810-11
AGTACTT438900.020.99107712-13
AGAGTAC683650.020.74979610-11
GTACTTT455650.020.43921914-15
Produced by FastQC (version 0.11.5)