FastQCFastQC Report
Thu 2 Jun 2016
DRR015254_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015254_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8684710
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401530.46234128715869616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350430.4035022470525786No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT236420.27222555502716844No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200290.23062370533961407No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT140140.16136405245540728RNA PCR Primer, Index 33 (95% over 22bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG104130.11990037663894362RNA PCR Primer, Index 33 (96% over 29bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88350.10173051259051828No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA237850.059.7927551
GTATCAA467300.043.561891
ATCAACG604900.033.416283
TCAACGC611100.033.0587274
TATCAAC620650.032.7223242
CAACGCA639850.031.5895545
AACGCAG660900.030.6936196
GTGGTAT83650.030.0987721
TGGTATC85350.028.2170012
ACGCAGA738800.027.2904057
CGCAGAG737700.027.1388448
GCAGAGT769250.025.4914349
GTACATG478800.023.2630751
TACATGG460800.023.039592
GAGTACT470550.022.40720712-13
CAGAGTA757100.021.53741310-11
ACATGGG483050.021.3751753
GTACTTT489350.021.21321514-15
AGAGTAC732350.020.75792310-11
AGTACTT475750.020.75083412-13
Produced by FastQC (version 0.11.5)