FastQCFastQC Report
Thu 2 Jun 2016
DRR015253_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015253_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9100378
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301400.3311950338766148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250150.27487869185214064No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146030.16046586196749188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA198700.056.69231
GTATCAA381900.043.349291
ATCAACG490950.033.3379753
TCAACGC495500.033.117434
TATCAAC510600.032.047942
CAACGCA535150.030.5616425
AACGCAG552600.029.6954126
GTGGTAT74400.028.7491421
ACGCAGA596300.027.2135267
CGCAGAG598700.026.9231058
TGGTATC81650.025.6451932
GCAGAGT629500.025.151069
GTACATG421050.023.8511121
TACATGG401450.023.6704942
ACATGGG429300.021.5436633
GAGTACT376750.021.429112-13
CAGAGTA617400.021.06399710-11
AGAGTAC596750.020.63025510-11
GTACTTT394000.020.26594514-15
GCCGTAT13700.020.2585590-91
Produced by FastQC (version 0.11.5)