FastQCFastQC Report
Thu 2 Jun 2016
DRR015253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9100378
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333350.36630346563626254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288240.31673409610018394No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT217220.2386933817474395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167570.1841352084495831No Hit
ATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTT120450.13235713945069094RNA PCR Primer, Index 23 (95% over 23bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG102580.11272059248527919RNA PCR Primer, Index 23 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA212950.058.8756681
GTATCAA409700.042.479671
ATCAACG526150.032.835763
TCAACGC530450.032.5579534
GTGGTAT75250.032.06181
TATCAAC543850.031.9761852
CAACGCA554200.031.1808385
AACGCAG576100.030.0824436
TGGTATC80750.028.639382
ACGCAGA641100.026.7979687
CGCAGAG642000.026.5417758
GCAGAGT677850.024.7039839
GTACATG433350.024.0378741
TACATGG416600.023.6363092
ACATGGG438600.021.7761783
GAGTACT414150.021.5224412-13
CAGAGTA664850.021.10456810-11
GTACTTT430200.020.40381214-15
AGAGTAC643950.020.23440410-11
AGTACTT414900.020.16687212-13
Produced by FastQC (version 0.11.5)