FastQCFastQC Report
Thu 2 Jun 2016
DRR015252_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015252_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9020487
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420750.466438231106591No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342170.37932541779617884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211750.2347434234980883No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA236350.055.514361
GTATCAA453300.043.9559171
ATCAACG596800.033.02393
TCAACGC602000.032.7039264
TATCAAC609200.032.422082
CAACGCA647100.030.4105365
AACGCAG662500.029.7723466
GTGGTAT91150.029.0196631
ACGCAGA720950.027.1347187
CGCAGAG729850.026.6733728
TGGTATC96250.026.6347392
GCAGAGT758950.025.3452079
GTACATG542000.023.0890391
TACATGG526800.022.4527532
GAGTACT430450.021.76797312-13
AGTACTT430650.021.2718912-13
CAGAGTA740900.021.07761410-11
AGAGTAC718550.020.81196810-11
GTACTTT445900.020.73858814-15
ACATGGG560450.020.6203063
Produced by FastQC (version 0.11.5)