FastQCFastQC Report
Thu 2 Jun 2016
DRR015252_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015252_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9020487
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT455870.5053718274855892No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT387870.4299878709431098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238000.26384384789867776No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT218810.2425700519273516No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT123180.13655582010150893RNA PCR Primer, Index 39 (95% over 23bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG103560.1148053314638112RNA PCR Primer, Index 39 (96% over 30bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96180.10662395500375978No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA265600.055.8824271
GTATCAA488850.042.497211
ATCAACG642600.032.0623473
TATCAAC655650.031.6533032
TCAACGC651300.031.625324
CAACGCA677400.030.455875
GTGGTAT99800.029.6528571
AACGCAG698300.029.5640546
TGGTATC104700.027.3479942
ACGCAGA771500.026.5226967
CGCAGAG781450.026.0502248
GCAGAGT813250.024.581689
GTACATG551400.023.6263081
TACATGG534100.023.012442
TACCTGG64700.023.0072652
GAGTACT476400.022.11589412-13
CAGAGTA793400.021.29943310-11
GTACTTT492300.021.21405214-15
AGTACTT477600.021.20727212-13
ACATGGG570000.021.0359633
Produced by FastQC (version 0.11.5)