FastQCFastQC Report
Thu 2 Jun 2016
DRR015251_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015251_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6755499
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT365110.540463406182134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288260.4267042301390319No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180040.26650881008197913No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68800.10184295786292026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA199550.055.156861
GTATCAA370750.041.670431
ATCAACG479250.031.9427643
TATCAAC479200.031.8944782
TCAACGC481200.031.758684
CAACGCA500550.030.4309275
AACGCAG515700.029.6342646
TACCTGG56750.027.5750142
ACGCAGA572300.026.3682737
TATAACG7100.026.3458982
GTGGTAT77650.026.2579021
CGCAGAG581250.025.8225178
GCAGAGT603100.024.5778689
GTACATG400100.024.511161
TGGTATC80050.024.507252
TACATGG392000.024.0221082
GAGTACT350400.022.26140812-13
ACATGGG413950.022.1472263
CATGGGT91100.021.8873144
AGTACTT353250.021.06433112-13
Produced by FastQC (version 0.11.5)