FastQCFastQC Report
Thu 2 Jun 2016
DRR015251_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015251_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6755499
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT408550.6047665760886057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343640.5086818901164814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209390.30995489748425686No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT139690.20677969162603682No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80940.11981350304396464No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT79080.11706019052034498TruSeq Adapter, Index 1 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA210900.054.8754581
GTATCAA390550.041.297291
ATCAACG509250.031.5364993
TCAACGC511450.031.4249824
TATCAAC513300.031.4118292
CAACGCA527600.030.5166285
AACGCAG546250.029.5580656
GTGGTAT81250.028.5665341
TATAACG5800.026.6849782
TACCTGG54000.026.6384662
TGGTATC86900.025.9823132
ACGCAGA617150.025.977957
CGCAGAG626100.025.450638
GTACATG395450.024.5266951
GCAGAGT649500.023.9600459
TACATGG388050.023.9191342
ACATGGG405550.022.0900443
CATGGGT86750.022.089874
GAGTACT395500.021.37929712-13
CAGAGTA630950.020.67983810-11
Produced by FastQC (version 0.11.5)