FastQCFastQC Report
Thu 2 Jun 2016
DRR015250_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015250_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9781658
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT355820.36376246235556386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291580.2980885244607816No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165730.16942935441006013No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA100540.10278421102025853No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA238750.055.253091
GTATCAA456300.043.305161
ATCAACG573900.034.0816463
TCAACGC583800.033.529154
TATCAAC585850.033.4188732
CAACGCA642650.030.485
AACGCAG661200.029.6593066
ACGCAGA696450.027.926147
CGCAGAG696600.027.6789028
GTGGTAT92700.026.2270321
GCAGAGT730350.025.8643869
TGGTATC97550.024.3685762
GTACATG480950.023.4829331
TACATGG467050.022.8890952
GAGTACT425600.022.3098512-13
CAGAGTA715400.021.33933310-11
ACATGGG488700.021.3390053
AGTACTT429650.020.98769212-13
GTACTTT447150.020.90932314-15
AGAGTAC683100.020.66997710-11
Produced by FastQC (version 0.11.5)