FastQCFastQC Report
Thu 2 Jun 2016
DRR015250_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015250_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9781658
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT374780.3831456793930027No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT329360.3367118335153406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321600.3287786181033931No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT184860.18898636611502875RNA PCR Primer, Index 27 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181240.18528556201821816No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG152550.15595515606863375RNA PCR Primer, Index 27 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCT101250.10351005933758879RNA PCR Primer, Index 27 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA246150.056.507091
GTATCAA468750.042.1745831
ATCAACG599200.032.8877033
TCAACGC608500.032.3839044
TATCAAC612250.032.2757642
CAACGCA648600.030.383625
AACGCAG666300.029.6316036
GTGGTAT92050.029.230161
TGGTATC95550.027.3938262
ACGCAGA721150.027.0552167
CGCAGAG724200.026.715118
GCAGAGT760600.024.871349
GTACATG495850.022.7077311
TACATGG481450.022.285582
GAGTACT445000.021.66146712-13
CAGAGTA745900.021.1548810-11
AGAGTAC713200.020.6121810-11
ACATGGG504950.020.5633893
GTACTTT469900.020.21537814-15
AGTACTT451100.020.21308312-13
Produced by FastQC (version 0.11.5)