FastQCFastQC Report
Thu 2 Jun 2016
DRR015249_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015249_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8579406
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT369330.43048434821711434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296950.3461195332171015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178980.2086158412365611No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA214100.056.3490681
GTATCAA417250.043.5512581
ATCAACG535350.033.4894143
TCAACGC541100.033.1526834
TATCAAC552500.032.56672
CAACGCA577050.031.0636185
AACGCAG594400.030.233836
GTGGTAT82150.028.8717751
TGGTATC84800.027.5988312
ACGCAGA645750.027.5468247
CGCAGAG652750.027.057218
GCAGAGT682500.025.5444899
GTACATG442850.023.7921161
TACATGG419750.023.5199132
GAGTACT400950.021.90541312-13
ACATGGG441000.021.467633
CAGAGTA662250.021.1452810-11
GTACTTT413300.020.96476414-15
AGTACTT402700.020.6296112-13
AGAGTAC639150.020.61401410-11
Produced by FastQC (version 0.11.5)