FastQCFastQC Report
Thu 2 Jun 2016
DRR015249_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015249_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8579406
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399310.465428492368819No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338320.39433965474999083No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204560.23843142520589422No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT198930.2318691993361778No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT129350.15076801354312874RNA PCR Primer, Index 26 (100% over 22bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG95790.111651086333949RNA PCR Primer, Index 26 (100% over 29bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA227800.058.5368161
GTATCAA441150.043.2111131
ATCAACG578600.032.7450373
TCAACGC583200.032.461644
TATCAAC593200.032.061892
CAACGCA606600.031.1821215
AACGCAG626450.030.281276
GTGGTAT81400.029.973321
ACGCAGA698200.026.9345577
TGGTATC88400.026.7261812
CGCAGAG701250.026.551378
GCAGAGT732350.024.867599
GTACATG447700.023.1637481
TACATGG429250.022.5356672
GAGTACT445250.021.82317212-13
AGTACTT448100.021.18150112-13
CAGAGTA709950.021.13915810-11
AGAGTAC689050.020.96448710-11
GTACTTT460050.020.91483914-15
ACATGGG458350.020.4593493
Produced by FastQC (version 0.11.5)