FastQCFastQC Report
Thu 2 Jun 2016
DRR015248_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015248_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8384486
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328600.39191430458587445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271350.3236334344168504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156350.18647535460134348No Hit
CATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTCGCCGT121760.14522058955074885Illumina Single End PCR Primer 1 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200000.055.7733571
GTATCAA379600.042.636361
ATCAACG489150.032.8736423
TCAACGC492450.032.6653254
TATCAAC506550.031.7702752
CAACGCA517950.030.9604595
AACGCAG534450.030.0557216
GTGGTAT78350.027.18181
ACGCAGA590600.026.9143457
CGCAGAG597650.026.5443678
TGGTATC81100.025.4795682
GCAGAGT629350.024.846049
GTACATG428100.024.6287821
TACATGG409000.024.068162
ACATGGG429000.022.4175683
TATAACG7300.021.870562
GAGTACT383450.021.42231812-13
CAGAGTA621300.020.92063910-11
AGTACTT379400.020.82357212-13
GTACTTT390800.020.73493414-15
Produced by FastQC (version 0.11.5)