FastQCFastQC Report
Thu 2 Jun 2016
DRR015248_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015248_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8384486
Sequences flagged as poor quality0
Sequence length97
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358400.4274561374424145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308230.36761943427420596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177580.21179592881424097No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT151390.1805596669849529No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT85840.10237956149011401RNA PCR Primer, Index 17 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA214250.055.9956931
GTATCAA410150.042.9859471
ATCAACG522450.033.4610253
TCAACGC525750.033.2393654
TATCAAC538850.032.6116072
CAACGCA545650.032.05315
AACGCAG563200.031.078726
ACGCAGA628450.027.6341087
CGCAGAG637550.027.176398
GTGGTAT80100.027.1647421
GCAGAGT665350.025.69489
TGGTATC84350.025.2588462
GTACATG431800.024.9737971
TACATGG411600.024.5102162
TATAACG7050.022.6012122
ACATGGG431700.022.3988253
AAACTCG16450.022.1387716
GAGTACT423500.022.02457812-13
CAGAGTA655050.021.67014710-11
ATCGTAT11250.021.4433759
Produced by FastQC (version 0.11.5)