FastQCFastQC Report
Thu 2 Jun 2016
DRR015247_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015247_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7906104
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT391230.49484550165290014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319810.40451023664753205No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190070.24040918257589328No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA112400.1421686332484369No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA108810.13762783793382935No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA86870.1098771278495704No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA230450.058.3101961
GTATCAA456050.043.7876631
ATCAACG597100.033.1313443
TCAACGC601100.032.874884
TATCAAC627700.031.6334482
GTGGTAT80650.030.9365181
CAACGCA659450.029.9798245
AACGCAG674650.029.3652886
TGGTATC85500.028.7628822
ACGCAGA703550.027.7922087
CGCAGAG711000.027.3617888
GCAGAGT743200.025.8950399
GTACATG573350.025.4099371
TACATGG544550.025.1609672
ACATGGG591000.022.6518633
GAGTACT419700.021.78359412-13
GTACTTT416900.021.67896514-15
AGTACTT411250.021.65037712-13
CAGAGTA729950.021.55946510-11
CATGGGG285300.020.9819164
Produced by FastQC (version 0.11.5)