FastQCFastQC Report
Thu 2 Jun 2016
DRR015247_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015247_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7906104
Sequences flagged as poor quality0
Sequence length97
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT437320.5531422303577084No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT367740.46513428105676324No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT265080.3352852428958688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218410.2762549038059707No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT141430.17888709786767287TruSeq Adapter, Index 11 (95% over 21bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG113630.14372439320302388TruSeq Adapter, Index 11 (96% over 28bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89960.11378550041841089No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA243650.059.0394441
GTATCAA492300.044.4505771
ATCAACG648250.033.65553
TCAACGC654100.033.3994144
GTGGTAT85650.032.4825021
TATCAAC681100.032.1793252
CAACGCA690200.031.7194635
TGGTATC87150.031.2403262
AACGCAG706200.031.0525786
ACGCAGA773950.028.1330597
CGCAGAG782650.027.7537488
GCAGAGT816050.026.2655499
TACATGG562050.024.9978542
GTACATG591750.024.9874381
ACATGGG607650.022.3427123
GAGTACT476050.022.07904212-13
GTACTTT475350.021.87464314-15
TACCTGG42800.021.8067952
AGTACTT465200.021.8062912-13
CAGAGTA803800.021.59270710-11
Produced by FastQC (version 0.11.5)