FastQCFastQC Report
Thu 2 Jun 2016
DRR015245_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015245_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8154120
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286080.35084104722520637No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240700.2951881993397203No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147170.18048544784722323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA160450.057.2939831
GTATCAA303300.040.958961
ATCAACG380850.032.3979643
GTGGTAT61950.032.3293461
TCAACGC383100.032.255194
TATCAAC389850.031.6644252
CAACGCA401750.030.7106575
AACGCAG419550.029.5582566
TGGTATC66100.028.6359272
GTACATG353600.026.5912911
ACGCAGA471550.025.9798877
CGCAGAG473850.025.7267678
TACATGG356600.025.2895852
ACATGGG366400.023.85363
GCAGAGT504650.023.642539
GAGTACT315700.021.1099112-13
CAGAGTA490550.020.78637710-11
GTACTTT331450.019.79829414-15
AGAGTAC473800.019.78339810-11
CATGGGG196550.019.5830064
Produced by FastQC (version 0.11.5)