FastQCFastQC Report
Thu 2 Jun 2016
DRR015245_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015245_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8154120
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344080.4219707338130908No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT282530.3464874198564652No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181080.22207178702300187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA170900.058.0394251
GTATCAA327500.042.1454621
ATCAACG413100.033.0024873
TCAACGC417150.032.684484
GTGGTAT66500.032.5595361
TATCAAC424400.032.2342262
CAACGCA432650.031.539375
AACGCAG452150.030.3715446
TGGTATC68950.029.325232
ACGCAGA518250.026.2879967
GTACATG358450.026.0948621
CGCAGAG519650.026.077028
TACATGG361100.024.8950142
GCAGAGT556650.024.0166179
ACATGGG366900.023.5930733
TATAACG7200.022.1374842
GAGTACT365000.021.1004112-13
CAGAGTA548950.020.46456310-11
AGAGTAC525900.020.1324510-11
TACCTGG63950.020.0817382
Produced by FastQC (version 0.11.5)