FastQCFastQC Report
Thu 2 Jun 2016
DRR015244_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015244_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6019054
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161240.2678826274029108No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140170.23287712653848927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82530.13711456983107312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102050.055.6079371
GTATCAA193900.039.288741
ATCAACG237850.031.7133333
TCAACGC239550.031.5067424
TATCAAC247300.030.5229242
TGGTATC41450.030.0766832
CAACGCA252000.029.9484165
AACGCAG265200.028.5766566
GTGGTAT46050.028.5293751
CGCAGAG289350.026.0168978
ACGCAGA293350.025.7095177
ACAACGC11500.024.9231683
GCAGAGT313150.023.5745549
TCTGTCG16100.023.1669588
GTACATG168200.022.7001551
TACATGG164050.021.7996862
GTCTCGC16500.020.7351281
GAGTACT228450.020.31050712-13
CAGAGTA315450.020.15964710-11
ACATGGG171750.020.1315573
Produced by FastQC (version 0.11.5)