FastQCFastQC Report
Thu 2 Jun 2016
DRR015244_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015244_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6019054
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179690.2985352847806317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159660.26525763018574017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95150.15808131975556292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105850.057.2340661
GTATCAA203800.040.39561
GTGGTAT44050.032.804531
ATCAACG250150.032.3261073
TGGTATC42100.032.2355772
TCAACGC253200.031.9740074
TATCAAC263850.030.809342
CAACGCA263900.030.6960245
TATAACG5550.030.3605752
AACGCAG276650.029.431596
ACAACGC8650.027.3714753
CGCAGAG307250.026.2790818
ACGCAGA310700.026.0312277
GTACATG169000.024.2759551
GCAGAGT331250.024.0728039
TACATGG162900.023.8467482
TCTGTCG16500.023.7226378
ACATGGG167650.022.3243683
ATAACGC8400.021.6816163
AGAGTAC319850.020.41991810-11
Produced by FastQC (version 0.11.5)