FastQCFastQC Report
Thu 2 Jun 2016
DRR015243_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015243_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6594709
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315120.4778376119401175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262610.39821317362145925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165230.25054934190424477No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78810.11950489399911353No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67670.10261256410252523No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA157200.056.426221
GTATCAA288550.040.5130841
GTGGTAT60000.031.7118851
ATCAACG370950.031.4653633
TCAACGC374550.031.1748454
TATCAAC383250.030.5414162
TGGTATC60300.030.1886062
CAACGCA392750.029.7279475
AACGCAG410300.028.5757586
ACGCAGA453250.025.5961157
CGCAGAG454750.025.4095158
GTACATG307900.024.600031
TACATGG302000.024.2314532
GCAGAGT479750.023.686079
ACATGGG322300.021.8983923
CAGAGTA473200.020.74211110-11
GAGTACT314350.020.62369312-13
TACCTGG42600.020.1907562
GTACTTT323000.019.88199214-15
AGAGTAC454600.019.86451510-11
Produced by FastQC (version 0.11.5)