FastQCFastQC Report
Thu 2 Jun 2016
DRR015243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6594709
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT368700.5590845630944443No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306910.4653882377524164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196560.2980571242794792No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80460.12200689977374286No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA68260.10350722071284724No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA165700.058.137391
GTATCAA316950.041.757531
ATCAACG407000.032.144143
TCAACGC412000.031.7132024
GTGGTAT66850.031.7124711
TGGTATC66100.031.2133062
TATCAAC422100.031.1727562
CAACGCA429250.030.5171035
AACGCAG444100.029.5289766
ACGCAGA500250.026.0244357
CGCAGAG503750.025.7351868
GTACATG321800.024.7080761
TACATGG315650.024.2407762
GCAGAGT531200.024.0025529
TATAACG6650.022.6013982
ACATGGG328700.022.2210853
GAGTACT358000.020.61076412-13
TACCTGG41550.020.6077162
CAGAGTA524950.020.59434710-11
AGAGTAC504700.020.19403610-11
Produced by FastQC (version 0.11.5)