FastQCFastQC Report
Thu 2 Jun 2016
DRR015242_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015242_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5489920
Sequences flagged as poor quality0
Sequence length97
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191720.3492218465842854No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166970.3041392224294707No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100750.18351815691303333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98250.055.6443521
GTATCAA184000.040.8481641
ATCAACG232400.031.9333523
TCAACGC234800.031.6841744
TATCAAC238950.031.0625462
CAACGCA244450.030.5611825
GTGGTAT39600.029.616571
AACGCAG257850.029.182586
TACCTGG43450.026.917422
ACGCAGA285100.026.2001347
TGGTATC42550.026.0963862
CGCAGAG285800.026.0706318
TATAACG5500.025.6501032
GTACATG165950.024.8592471
GCAGAGT303150.023.8128479
GTACCTG51550.023.6362951
TACATGG166500.023.1231082
TAACGCA7000.022.7505934
GTACCGG7050.022.655611
ACAACGC11050.022.2360783
Produced by FastQC (version 0.11.5)