FastQCFastQC Report
Thu 2 Jun 2016
DRR015242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5489920
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223020.40623542783865707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188550.3434476276521334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119200.2171252040102588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109050.055.1896441
GTATCAA199800.039.7522771
ATCAACG250100.031.2254643
TCAACGC252900.030.8639954
GTGGTAT44100.030.4995021
TATCAAC258300.030.4168532
CAACGCA261650.029.8495125
AACGCAG272100.028.889776
TACCTGG50200.028.7610762
GTACCTG54450.027.4653591
TGGTATC48050.026.825182
ACGCAGA306150.025.4701257
CGCAGAG309300.025.0929958
TATAACG6000.025.0502622
GTACATG169400.023.8467731
GCAGAGT326600.023.38759
ACAACGC9300.023.0130083
ACCTGGG63000.022.6236293
TACATGG168150.022.129662
ACATGGG168600.021.3097293
Produced by FastQC (version 0.11.5)