FastQCFastQC Report
Thu 2 Jun 2016
DRR015241_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015241_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5577566
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170860.3063343401046263No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138830.24890785693974754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89170.15987260392794994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89350.056.683511
GTATCAA172600.040.7106671
ATCAACG214950.032.302663
TCAACGC216950.031.9629274
GTGGTAT38350.031.1719681
TATCAAC223100.031.1678922
CAACGCA227550.030.5504065
AACGCAG239800.029.1217866
TGGTATC38200.028.7102722
ACGCAGA266350.026.0974627
CGCAGAG266100.025.9156678
GCAGAGT283700.023.4572739
GTACATG147900.023.4154151
TACATGG146150.022.5747432
TCTGTCG13100.021.8764748
TATAACG5650.021.7469792
ACAACGC9900.021.6014983
ACATGGG150650.020.477733
GTCTCGC14350.020.3496231
AGAGTAC270650.020.32944110-11
Produced by FastQC (version 0.11.5)