FastQCFastQC Report
Thu 2 Jun 2016
DRR015241_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015241_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5577566
Sequences flagged as poor quality0
Sequence length97
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203810.3654102882870413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173330.31076279509735966No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107520.1927722594407668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106200.059.839811
GTATCAA202850.041.194521
GTGGTAT40800.034.1911131
TGGTATC39150.034.084032
ATCAACG251200.032.8820343
TCAACGC254600.032.427364
TATCAAC262650.031.6099872
CAACGCA263700.031.3978465
AACGCAG276200.030.060086
ACAACGC9300.027.4186483
ACGCAGA308700.026.6018757
CGCAGAG308700.026.4101778
GCAGAGT328800.024.408049
TACCTGG36950.022.9252222
GTACATG150050.021.4496751
GAGTACT254200.021.05912212-13
CAGAGTA330450.021.02826310-11
GTCTCGC14400.020.5779841
AGAGTAC315500.020.4087310-11
TACATGG150250.020.1871412
Produced by FastQC (version 0.11.5)