FastQCFastQC Report
Thu 2 Jun 2016
DRR015240_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR015240_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9641253
Sequences flagged as poor quality0
Sequence length97
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT257060.26662509530659556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT213340.22127829235473853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129280.13409045484025778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164050.054.147941
GTATCAA308750.040.6809841
ATCAACG383550.032.3765143
TCAACGC388500.031.9288584
TATCAAC405350.030.6715132
CAACGCA407850.030.467285
AACGCAG427050.029.2659786
ACGCAGA476800.025.9144957
CGCAGAG478100.025.6621728
GTACATG350500.024.9011341
GTGGTAT71600.024.6598341
TACATGG341100.024.0146062
GCAGAGT509250.023.654379
TGGTATC72100.023.1009852
ACATGGG354550.022.3316233
GAGTACT318250.020.47780212-13
CAGAGTA502850.020.13917210-11
AGAGTAC478550.020.00199510-11
AGTACTT324700.019.41950212-13
GTACTTT333500.019.33678814-15
Produced by FastQC (version 0.11.5)